Technologies for all-optical interrogation of neural circuits in behaving animalsTechnologies for all-optical interrogation of neural circuits in behaving animals
Date:
Lecture / Seminar
Time: 12:30
Lecturer: Dr. Adam Packer
Organizer: Department of Brain Sciences
Details: Department of Physiology, Anatomy and Genetics
University of Oxford, UK
Abstract: Neural circuits display complex spatiotemporal patterns of activity on the milli ... Read more Neural circuits display complex spatiotemporal patterns of activity on the millisecond timescale during behavior. Understanding how these activity patterns drive behavior is a fundamental problem in neuroscience, and remains a major challenge due to the complexity of their spatiotemporal dynamics. The ability to manipulate activity in genetically defined sets of neurons on the millisecond timescale using optogenetics has provided a powerful new tool for making causal links between neuronal activity and behavior. I will discuss novel approaches that combine simultaneous two-photon calcium imaging and two-photon targeted optogenetic photostimulation with the use of a spatial light modulator (SLM) to provide ‘all-optical’ readout and manipulation of the same neurons in vivo. This approach enables reading and writing of activity in neural circuits with single-cell resolution and single action potential precision during behavior. I will describe the power, limitations and future potential of this approach; and discuss how it can be used to address many important problems in neuroscience, including transforming our search for the neural code and the links between neural circuit activity and behavior.
Zoom link to join-
https://weizmann.zoom.us/j/96608033618?pwd=SEdJUkR2ZzRBZ3laUUdGbWR1VFJTdz09
Meeting ID: 966 0803 3618
Password: 564068
Host: Dr. Rita Schmidt rita.schmidt@weizmann.ac.il tel: 9070
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