Fast protein liquid chromatography (FPLC)
Fast protein liquid chromatography such as the latest-generation AKTA Avant 25 are used for protein purification. Using columns that separate proteins according to their specific affinity, size, charge, hydrophobicity… a protein can be purified from the protein mixture (cell lysate). The unit holds a wide variety of chromatographic columns.
Microplate Readers
Multi-Mode Microplate readers measure transmittance, fluorescence and luminescence in microplate (96-well, 384-well…) format, in volumes ranging from several milliliters to several microliters. The plate readers are equipped with two injectors, allowing automated injection of material to start the reaction, thus increasing accuracy of measurements especially in kinetic mode. Other assays available are: Fluorescence Polarization, Time-Resolved Fluorescence (including TR-FRET), AlphaScreen/AlphaLISA.
Fluorescence Laser Scanner
Typhoon FLA 9500 Biomolecular Imager is a versatile, state-of-the-art laser scanner suited for applications such as sensitive and quantitative measurements of multiplex fluorescence and radioisotopic labels. It scans at a resolution of up to 10 μm using three lasers (473nm, 532nm, 6355nm) and matching filters (>510nm, >575nm, >665nm, 520-540nm, 560-580nm).
Polymerase Chain Reaction (PCR) instruments
PCR amplifies exact DNA sequences. It can also be used to create specific changes in DNA sequences (site directed mutagenesis) and insert DNA sequences into specific sites (restriction free cloning). A Real Time or quantitative PCR instrument allows quantification of DNA template in the PCR mixture. Differential RNA/DNA expression is done by following in real time DNA amplification. For example: Real Time PCR can detect the presence of bacteria or viruses in a sample.