High content CyTOF-based screening for discovery of novel immune-receptor inhibitors for lymphoma treatment (CyTOF-screen)

B cells and B cell lymphoma depend on BCR signaling for their survival. Removal or inhibition of the immune receptor signaling lead to elimination of the malignant cell. Thus, immune-receptor signaling pathways are an excellent target for cancer treatment. We aim to establish a large-scale screening method that generates numerous readouts in response to hundreds of perturbations by combing high throughput screening with CyTOF readouts.  This would allow to define the effect of each treatment on immune cells and lymphoma cells from patients and examine multiple parameters readout by CyTOF.